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Heh no (that would be illegal) - it's from this old cartoon called Cow & Chicken, from Cartoon Network. It was supposedly for children, but it was incredibly dark and mature. It's a sarcastic way of expressing being surprised, kind of like "You don't say..." in a sarcastic tone.
The full quote: "Well paint me red and call me a girl scout."
Or better yet, shave my legs and call me grandpa!
Yeah, the question - how on earth could that cartoon be aired on CN, leaves me wondering up to this very day.